IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/29: Difference between revisions

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==Team Allergy==
==Team Allergy==
* Rules the world.
 
* Also, we ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
* Ran a gel for our second attempt at amiRNA stiching (stage 1): [[Image:AmiRNA_stich2_stage1.jpg|50px]]. It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
 
*Dignostic Digest
'''Gel:'''
ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK
 
'''Expected band lengths:'''
 
''Complete Parts''
9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp
25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp
36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp
 
''Sense + Intron Parts''
LTPS+ PDK: 1200 and 3200 bp
Bet2S+ PDK: 1200 and 3200 bp
GerS+PDK: 1200 and 3200 bp
 
[[Image: |240 px]]


==Team Flavor==
==Team Flavor==

Revision as of 08:57, 29 July 2010

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Team Allergy

  • Ran a gel for our second attempt at amiRNA stiching (stage 1): . It seems that the backbone is still present at high concentrations after PCR purification. Maybe this is why last time we had a high incidence of background? This time we're gel purifying.
  • Dignostic Digest

Gel: ladder, 9, 9, 11, 11, 25, 25, 28, 28, ladder, 36, 36, 37, 37, 38, 38, ladder, LTPS+ PDK, LTPS+PDK, Bet2S+PDK, Bet2S+PDK, GerS+PDK, GerS+PDK, GerS+PDK, GerS+PDK

Expected band lengths:

Complete Parts 9,11 (LTPS+Pal+LTPA): 840bp and 3200 bp 25,28 (Bet2S+Pal+Bet2A): 840bp and 3200 bp 36,37,38 (GerS+Pal+GerA): 840bp and 3200 bp

Sense + Intron Parts LTPS+ PDK: 1200 and 3200 bp Bet2S+ PDK: 1200 and 3200 bp GerS+PDK: 1200 and 3200 bp

[[Image: |240 px]]

Team Flavor

Minipreps

  • Miniprep of Mira/Brazz+StrepII+STOP in V0120

Gel Extraction

  • Gel extraction of J45017: upcoming



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