IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/30: Difference between revisions
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Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify. | Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify. | ||
===Second ACC + RxrHm digest gel=== | |||
[[Image:sucesspcrkfkdigestacvtually.jpg]] | [[Image:sucesspcrkfkdigestacvtually.jpg]] | ||
yes to ACC (right) no RxrHm | |||
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Revision as of 15:29, 1 August 2010
 iGEM iGarden
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Team Allergy
LTP ihpRNA parts that worked: 9c2, 11c1, 11c2 Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA) Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2 Team Flavor
Ligations were plated and left @ 37°C O/N. Team FencePCR of VP16 and Gal4DBD
PCR again for VP16Using pfx polymerase
Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify. Second ACC + RxrHm digest gel
yes to ACC (right) no RxrHm
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