IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/07/30: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 70: Line 70:
[[Image:sucesspcrkfkdigestacvtually.jpg]]
[[Image:sucesspcrkfkdigestacvtually.jpg]]


yes to ACC (right) no RxrHm
yes to ACC (left) no RxrHm
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->


Revision as of 15:29, 1 August 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Allergy

  • Sequencing Results:

LTP ihpRNA parts that worked: 9c2, 11c1, 11c2

Bet 2 ihpRNA parts that worked: 25c1,c2, 28c1,(LTPS+PDK is actually Bet 2 hpRNA)

Ger ihpRNA parts that worked: 36c1,c2, 37c1,2, 38c1,c2

Team Flavor

  • Ligated StrepII tagged proteins into V24 backbone
  • Ligated cut J45004 into V0120 backbone

Ligations were plated and left @ 37°C O/N.

Team Fence

PCR of VP16 and Gal4DBD

1 2 3 4 5 6 7 8 9 10 11 12
KB+ Ladder Gal4 DBD 1 Gal4 DBD 2 Gal4 DBD 3 Gal4 DBD 4 VP16 1 VP16 2 VP16 3 VP16 4 KB+ Ladder

PCR again for VP16

Using pfx polymerase

  • 1μL 1/10 dilution Hind.Fwd
  • 1μL 1/10 dilution VP16.Rev
  • 1μL DNTPs
  • 6μL enhancer buffer
  • .5μL MgSO4
  • .5μL PFX polymerase
  • 1μL 1/10 dilution VP16 #1
  • 5μL DH2O
  • 4μL amp(lification?) buffer

Running PCR on two reactions. There was not enough PFX for both to get .5μL, so only one of them is likely to actually amplify.

Second ACC + RxrHm digest gel

yes to ACC (left) no RxrHm



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/07/30&oldid=438463"