IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02: Difference between revisions
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==Team Flavor== | ==Team Flavor== | ||
===DTL of Mira/Brazz+StrepII+Stop into V24=== | |||
* Digested 1 microgram of DNA for each insert and for V24 backbone. | * Digested 1 microgram of DNA for each insert and for V24 backbone. | ||
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* Gel extracted and purified bands indicated in image below | * Gel extracted and purified bands indicated in image below | ||
* Ligated insert into 50ng of V24 backbone with a 3:1 insert to backbone ratio | |||
* Transformed and plated on LB+AMP plates and left in 37°C incubator overnight | |||
===DTL of Wintergreen pathway=== | |||
* Digested 1 microgram of J45004 and 20ng of J45017 | |||
Revision as of 06:39, 3 August 2010
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Team FenceGel extractionGal4 PCR product and Ec digest gel extract and purified. Gal on the left, EcR on the right VP16 PCR 2No PCR product is visible
Team FlavorDTL of Mira/Brazz+StrepII+Stop into V24
DTL of Wintergreen pathway
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