IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02: Difference between revisions

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[[Image:VP16PCR.2_blank_8-2.jpg]]
[[Image:VP16PCR.2_blank_8-2.jpg]]
==Team Flavor==
''DTL of Mira/Brazz+StrepII+Stop into V24''
* Digested 1 microgram of DNA for each insert and for V24 backbone.
** Digested inserts and backbone with NotI/SpeI
* Gel extracted and purified bands indicated in image below


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Revision as of 06:33, 3 August 2010

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Team Fence

Gel extraction

Gal4 PCR product and Ec digest gel extract and purified.

Gal on the left, EcR on the right


VP16 PCR 2

No PCR product is visible


Team Flavor

DTL of Mira/Brazz+StrepII+Stop into V24

  • Digested 1 microgram of DNA for each insert and for V24 backbone.
    • Digested inserts and backbone with NotI/SpeI
  • Gel extracted and purified bands indicated in image below



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