IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/02: Difference between revisions
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* Digested 400ng of J45004, 20ng of J45017, and 1 microgram of B21 (for v0120 backbone) | * Digested 400ng of J45004, 20ng of J45017, and 1 microgram of B21 (for v0120 backbone) | ||
** Digested J45004, J45017, and B21 BB with XbaI/PstI | ** Digested J45004, J45017, and B21 BB with XbaI/PstI | ||
* Gel extracted and purified | * PCR purified digested inserts | ||
* Gel extracted and purified B21 BB (see gel image below) | |||
Revision as of 07:46, 3 August 2010
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Team FenceGel extractionGal4 PCR product and Ec digest gel extract and purified. Gal on the left, EcR on the right VP16 PCR 2No PCR product is visible
Team FlavorDTL of Mira/Brazz+StrepII+Stop into V24
DTL of Wintergreen pathway
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