IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 13: Line 13:
==Team Fence==
==Team Fence==


[[Image: 83gelpcrvp16.jpg]]
[[Image: 83gelpcrvp16.jpg||350px]]


PCR of VP16
===Yesterday's transformations===
 
[[Image:Gal4-EcR_lig_8-3.jpg]]


==Team Flavor==
==Team Flavor==

Revision as of 10:29, 3 August 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Allergy

  • Grew up cultures of completed ihpRNA in pORE expression vector (Bet2, LTP, Ger)
  • amiRNA PCR
    • PCR for each of the seperate parts seemed to have work

[[Image: |240px|PCR gel]] ladder,

Team Fence

Yesterday's transformations

Team Flavor

  • Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/08/03&oldid=439170"