IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/03

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(Team Flavor)
(Team Flavor)
Line 28: Line 28:
==Team Flavor==
==Team Flavor==
* Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C
* Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C
-
* Glycerol stocks were made (666μL glycerol: 333μL cells)
+
* Glycerol stocks were made (666μL glycerol: 333μL cells; 50% glycerol final)
* Miniprepped per Qiagen protocol
* Miniprepped per Qiagen protocol

Revision as of 16:16, 3 August 2010

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Team Allergy

  • Grew up cultures of completed ihpRNA in pORE expression vector (Bet2, LTP, Ger)
  • amiRNA PCR
    • PCR for each of the seperate parts seemed to have work

[[Image: |240px|PCR gel]] ladder,

Team Fence

VP16 PCR from last night

Yesterday's transformations

Team Flavor

  • Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C
  • Glycerol stocks were made (666μL glycerol: 333μL cells; 50% glycerol final)
  • Miniprepped per Qiagen protocol
   ODs
    004-1: 95.5 ng/μL
    004-2: 124.5 ng/μL
    017-1: 109.7 ng/μL
    017-2: 77.5 ng/μL
    Miraculin N+Strep+Stop: 61.5 ng/μL
    Miraculin C+Strep+Stop: 78.5 ng/μL
    Brazzein N+Strep+Stop: 83.4 ng/μL
    Brazzein C+Strep+Stop: 99.4 ng/μL
 


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