IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/03: Difference between revisions
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==Team Flavor== | ==Team Flavor== | ||
* Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C | * Colonies were obtained from all ligations; 5mL cultures were started, left ~5hrs @ 37°C | ||
* Glycerol stocks were made (666μL glycerol: 333μL cells) | * Glycerol stocks were made (666μL glycerol: 333μL cells; 50% glycerol final) | ||
* Miniprepped per Qiagen protocol | * Miniprepped per Qiagen protocol | ||
Revision as of 13:16, 3 August 2010
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Team Allergy
[[Image: |240px|PCR gel]] ladder, Team FenceYesterday's transformationsTeam Flavor
ODs 004-1: 95.5 ng/μL 004-2: 124.5 ng/μL 017-1: 109.7 ng/μL 017-2: 77.5 ng/μL Miraculin N+Strep+Stop: 61.5 ng/μL Miraculin C+Strep+Stop: 78.5 ng/μL Brazzein N+Strep+Stop: 83.4 ng/μL Brazzein C+Strep+Stop: 99.4 ng/μL |