IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/03
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Team Allergy
[[Image: |240px|PCR gel]] ladder, Team FenceYesterday's transformationsGlycerol Stocksfrom 7-27-10 keeping Act2lac0pt 1, ACC synthase 1, EcR 2, RXRLc 2 added .5mL 80% glycerol to .5mL overnight cell culture of selected transformation, vortexed thoroughly, and placed in the -80°C freezer in team fence box. Annealing 35s min promoter
placed eppendorf in boiling water for 2 mins, then removed water from heat and allowed water and eppendorf to slowly cool together. Team Flavor
ODs 004-1: 95.5 ng/μL 004-2: 124.5 ng/μL 017-1: 109.7 ng/μL 017-2: 77.5 ng/μL Miraculin N+Strep+Stop: 61.5 ng/μL Miraculin C+Strep+Stop: 78.5 ng/μL Brazzein N+Strep+Stop: 83.4 ng/μL Brazzein C+Strep+Stop: 99.4 ng/μL |