IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/04: Difference between revisions
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6=4.0°C forever | 6=4.0°C forever | ||
7=end | 7=end | ||
===Digestion of Gal-EcR=== | |||
BamH1 and Nco1 | |||
12 digestions, one for each Gal-EcR miniprep | |||
Each: | |||
*1μL BamH1 | |||
*1μL Nco1 | |||
*1μL 10x FD green buffer | |||
*2-7μL EcR-Gal miniprep | |||
*5-0μL DH<sub>2</sub>O, to bring total volume to 10μL | |||
Ran a 1% gel of the digest | |||
[[Image:EcR-Gal_lig_VP16.4_Pcr_8-04.jpg]] | [[Image:EcR-Gal_lig_VP16.4_Pcr_8-04.jpg]] |
Revision as of 14:26, 7 September 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Team Allergy
[[Image: |240 px]] (Should see ~ 7kb band and an 840 band)
[[Image: |240px|Gel 2]] Gel 1: Ladder, 9, 11c1, 11c2, ladder, 25c1, 28c1, 28c2, 36c1, 36c2, ladder Gel 2: Ladder, 37c1, 37 c2, 38c1, 38c2, ladder Team FencePCR of VP16.360μL reaction:
Using program: 1=94°C for 2:00 2=94°C for :15 3=60/68°C for :30 4=68°C for :10 5=Goto 2, 30 times 6=4.0°C forever 7=end Digestion of Gal-EcRBamH1 and Nco1 12 digestions, one for each Gal-EcR miniprep Each:
Ran a 1% gel of the digest Team FlavorConfirmation Digests
Retry Ligating j45004 and v0120
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