IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/04
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Team Allergy
[[Image: |240 px]] (Should see ~ 7kb band and an 840 band)
[[Image: |240px|Gel 2]] Gel 1: Ladder, 9, 11c1, 11c2, ladder, 25c1, 28c1, 28c2, 36c1, 36c2, ladder Gel 2: Ladder, 37c1, 37 c2, 38c1, 38c2, ladder Team FencePCR of VP16.360μL reaction:
Using program: 1=94°C for 2:00 2=94°C for :15 3=60/68°C for :30 4=68°C for :10 5=Goto 2, 30 times 6=4.0°C forever 7=end Digestion of Gal-EcRBamH1 and Nco1 12 digestions, one for each Gal-EcR miniprep Each:
Ran a 1% gel of the digest
Sending EcR-Gal for sequencing
Sealed tubes with parafilm. Did not submit Gal-EcR 4 on account of insufficient DNA to both sequence and use it. Ligated EcR to Gal4 and Barnase to StrepTeam FlavorConfirmation Digests
Retry Ligating j45004 and v0120
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