IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/05: Difference between revisions
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[[Image:V9v10.jpg|240px]] | [[Image:V9v10.jpg|240px]] | ||
* Verified that amiRNA stitching of Bet, LTP yielded the proper insert with a low level of background through PCR: | |||
[[Image:AmiRNA-insert-diagnostic.jpg|240px]] | |||
* Digested Bet,LTP inserts with X+P, B21 with X+P+phosphatase | |||
* Ligated, transformed | |||
** 0-7: Bet, corresponding to 65..55 degrees C for stitching Tm (even spacing) | |||
** 8-15: LTP, corresponding to 65.55 degrees C during stitching annealing, even spacing | |||
Revision as of 17:10, 5 August 2010
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