IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/05: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 10: | Line 10: | ||
==Team Allergy== | ==Team Allergy== | ||
* | * Gel extracte V9/V10 backbone | ||
[[Image:V9v10.jpg|240px]] | [[Image:V9v10.jpg|240px]] | ||
''Concentrations: V9 (9.4 ng/uL; V10 (16.4 ng/uL) | |||
*Ligated ihpRNA inserts into V9/V10 and transformed | |||
**For our ligations we only used ~ 2uL of backbone (around 18 and 32 ng of backbone)and used a 3x excess of insert | |||
* Verified that amiRNA stitching of Bet, LTP yielded the proper insert with a low level of background through PCR: | * Verified that amiRNA stitching of Bet, LTP yielded the proper insert with a low level of background through PCR: | ||
[[Image:AmiRNA-insert-diagnostic.jpg|240px]] | [[Image:AmiRNA-insert-diagnostic.jpg|240px]] |
Revision as of 08:20, 6 August 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Team FlavorTransformation of J45004
Team Allergy
Concentrations: V9 (9.4 ng/uL; V10 (16.4 ng/uL)
|