IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/06: Difference between revisions
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Brazz C1 | Brazz C1 | ||
Brazz C2 | Brazz C2 | ||
==Team Fence== | |||
===Digestions=== | |||
pACT2 | |||
*1μL Bam | |||
*1μL Xho | |||
*6μL pACT2 (tube with a green top) | |||
*1μL FD Green buffer | |||
*1μL DH<sub>2</sub>O | |||
RXRLc | |||
*1μL Bam | |||
*1μL Xho | |||
*9μL RXRLc4 | |||
*2μL FD Green buffer | |||
*7μL DH<sub>2</sub>O | |||
Barstar | |||
*1μL Eco | |||
*1μL Pst | |||
*2μL Barstar 3 | |||
*1μL FD Green buffer | |||
*5μL DH<sub>2</sub>O | |||
Barnase | |||
*1μL Eco | |||
*1μL Pst | |||
*1.5μL Barnase 2 | |||
*1μL FD Green buffer | |||
*5.5μL DH<sub>2</sub>O | |||
5xGalUAS | |||
*1μL Spe | |||
*1μL Pst | |||
*2μL 5xGalUAS (from Karmella) | |||
*1μL FD Green buffer | |||
*5μL DH<sub>2</sub>O | |||
pENT Cup | |||
*1μL Spe | |||
*1μL Pst | |||
*1μL pENT Cup | |||
*1μL FD Green buffer | |||
*6μL DH<sub>2</sub>O | |||
EcR-Gal | |||
*1μL Xba | |||
*1μL Pst | |||
*2μL EcR-Gal 7 | |||
*1μL FD Green buffer | |||
*5μL DH<sub>2</sub>O | |||
VP16 | |||
*1μL Spe | |||
*1μL Pst | |||
*5μL VP16(1) | |||
*1μL FD Green buffer | |||
*2μL DH<sub>2</sub>O | |||
RXRLc | |||
*1μL Xba | |||
*1μL Pst | |||
*9μL RXRLc4 | |||
*2μL FD Green buffer | |||
*7μL DH<sub>2</sub>O | |||
V24 | |||
*1μL Eco | |||
*1μL Pst | |||
*3μL V24 | |||
*1μL FD Green buffer | |||
*4μL DH<sub>2</sub>O | |||
V26 | |||
*1μL Eco | |||
*1μL Pst | |||
*9μL V26 | |||
*12μL FD Green buffer | |||
*7μL DH<sub>2</sub>O | |||
PhyB | |||
*1μL Bam | |||
*1μL Nco | |||
*16μL PhyB | |||
*2μL FD Green buffer | |||
Revision as of 15:02, 7 September 2010
iGEM iGarden | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Team AllergyamiRNA
ihpRNA
[[Image: |240px|V9 and V10 backbone]] Concentrations: V9 (5 ng/uL); V10 (6 ng/uL)
Team FlavorConfirmation Digest of J45004
Backbone: 3.2 kb; Insert: 1.1 kb AnalysisBoth the backbone and the insert in lane 1 look like the correct length (3.2 and 1.1 kb respectively). Lane two consists of a backbone of the correct length, but the insert appears to be ~1.5 kb, too long for the J45004 BioBrick part. Lane 3 appears undigested; we are uncertain as to why. Transformation of Mira/Brazz StrepII constucts into E45
Transformations: Mira N1 Mira N2 Mira C1 Mira C2 Brazz N1 Brazz N2 Brazz C1 Brazz C2
Team FenceDigestionspACT2
RXRLc
Barstar
Barnase
5xGalUAS
pENT Cup
EcR-Gal
VP16
RXRLc
V24
V26
PhyB
|