IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 14: Line 14:
**Redigested and gel purified some backbone
**Redigested and gel purified some backbone


==Team Flavor==
===Confirmation Digest of J45004===
*J45004 in V0120 backbone; digested ~500ng DNA with Xba1/Pst1
[[Image:J45004_confirm.jpg||240px]] [[Image:J45004_confirm2.jpg||240px]]
# Ladder
# 004-1
# 004-2
# 004-3
# Ladder
Backbone: 3.2 kb; Insert: 1.1 kb
====Analysis====
Both the backbone and the insert in lane 1 look like the correct length (3.2 and 1.1 kb respectively).  Lane two consists of a backbone of the correct length, but the insert appears to be ~1.5 kb, too long for the J45004 BioBrick part.  Lane 3 appears undigested; we are uncertain as to why.




Revision as of 09:00, 6 August 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Team Allergy

amiRNA

  • amiRNA-transformed turbo cells didn't grow in YEB medium. There should be more LB+Amp available by the end of the day. In the meantime, we're digesting some more backbone (8x20ul X+P+B21)

ihpRNA

  • no colonies on plates from transformations done yesterday after 16 hours (we let these plates grow for longer)
  • redoing ligations of ihpRNA inserts in V9/V10
    • Redigested and gel purified some backbone

Team Flavor

Confirmation Digest of J45004

  • J45004 in V0120 backbone; digested ~500ng DNA with Xba1/Pst1

  1. Ladder
  2. 004-1
  3. 004-2
  4. 004-3
  5. Ladder

Backbone: 3.2 kb; Insert: 1.1 kb

Analysis

Both the backbone and the insert in lane 1 look like the correct length (3.2 and 1.1 kb respectively). Lane two consists of a backbone of the correct length, but the insert appears to be ~1.5 kb, too long for the J45004 BioBrick part. Lane 3 appears undigested; we are uncertain as to why.



Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/08/06&oldid=440518"