IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/09: Difference between revisions
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==Team Fence== | ==Team Fence== | ||
Gel Extracted Friday's Digests | |||
===Nanodrop of Friday's Digest Gel Extractions=== | |||
{| border="3" style="margin-left: 3em;" | |||
|- | |||
! Plasmid !! Quantity (ng/μL) !! 260/280 | |||
|- | |||
! Barstar Eco Pst 1 | |||
| .9 || -.57 | |||
|- | |||
! Barstar Eco Pst 2 | |||
| -.4 || .14 | |||
|- | |||
! RXRLc Xba Pst | |||
| .3 || -.19 | |||
|- | |||
! Barnase Eco Pst 1 | |||
| 20.5 || 1.64 | |||
|- | |||
! Barnase Eco Pst 2 | |||
| 14.4 || 1.83 | |||
|- | |||
! 5xGalUAS Spe Pst 1 | |||
| 10.7 || 1.87 | |||
|- | |||
! 5xGalUAS Spe Pst 2 | |||
| 16.4 || 1.90 | |||
|- | |||
! pENTcup Spe Pst 1 | |||
| 16.3 || 1.88 | |||
|- | |||
! pENTcup Spe Pst 2 | |||
| 14.7 || 2.09 | |||
|- | |||
! PhyB Bam Nco 1 | |||
| 1.7 || -1.10 | |||
|- | |||
! PhyB Bam Nco 2 | |||
| -.6 || .24 | |||
|- | |||
! V26 Eco Pst 1 | |||
| 2.4 || 1.6 | |||
|- | |||
! V26 Eco Pst 2 | |||
| 1.3 || 1.05 | |||
|- | |||
! V26 Eco Pst 3 | |||
| 1.4 || 3.01 | |||
|- | |||
! V24 Eco Pst 1 | |||
| 8.3 || 3.6 | |||
|- | |||
! V24 Eco Pst 2 | |||
| 5.5 || 1.64 | |||
|- | |||
! VP16 Eco Pst 1 | |||
| 3.4|| 1.6 | |||
|- | |||
! VP16 Spe Pst 1 | |||
| 9.4 || 1.92 | |||
|- | |||
! VP16 Spe Pst 2 | |||
| 36.9 || 1.73 | |||
|- | |||
! RXRLc Bam Xho 1 | |||
| 7.7 || 2.51 | |||
|- | |||
! RXRLc Bam Xho 2 | |||
| 4.1 || 1.93 | |||
|- | |||
! 35s min anneal 1/100 from 8/2 | |||
| 3.6 || 1.31 | |||
|- | |||
! RXRLc Xba Pst | |||
| 3.6 || 1.31 | |||
|- | |||
|} | |||
Redo of some digests with no insert where there should have been an insert | Redo of some digests with no insert where there should have been an insert | ||
[[Image: 89moredigestspm.jpg]] | [[Image: 89moredigestspm.jpg]] | ||
*ladder | |||
*pACT2 (backbone) | |||
*EcRGal (insert)- STILL NO INSERT | |||
*RxRLC (insert) Xba and Pst | |||
*RxRLC (insert) Bam and Xho | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
__NOTOC__ | __NOTOC__ |
Revision as of 15:19, 7 September 2010
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Team Allergy
Team FenceGel Extracted Friday's Digests Nanodrop of Friday's Digest Gel Extractions
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