IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/09: Difference between revisions

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===Nanodrop of Friday's Digest Gel Extractions===
===Nanodrop of Friday's Digest Gel Extractions===
{| border="3" style="margin-left: 3em;"
|-
! Plasmid !! Quantity (ng/μL) !! 260/280
|-
! Barstar Eco Pst 1
| .9 || -.57
|-
! Barstar Eco Pst 2
| -.4 || .14
|-
! RXRLc Xba Pst
| .3 || -.19
|-
! Barnase Eco Pst 1
| 20.5 || 1.64
|-
! Barnase Eco Pst 2
| 14.4 || 1.83
|-
! 5xGalUAS Spe Pst 1
| 10.7 || 1.87
|-
! 5xGalUAS Spe Pst 2
| 16.4 || 1.90
|-
! pENTcup Spe Pst 1
| 16.3 || 1.88
|-
! pENTcup Spe Pst 2
| 14.7 || 2.09
|-
! PhyB Bam Nco 1
| 1.7 || -1.10
|-
! PhyB Bam Nco 2
| -.6 || .24
|-
! V26 Eco Pst 1
| 2.4 || 1.6
|-
! V26 Eco Pst 2
| 1.3 || 1.05
|-
! V26 Eco Pst 3
| 1.4 || 3.01
|-
! V24 Eco Pst 1
| 8.3 || 3.6
|-
! V24 Eco Pst 2
| 5.5 || 1.64
|-
! VP16 Eco Pst 1
| 3.4|| 1.6
|-
! VP16 Spe Pst 1
| 9.4 || 1.92
|-
!  VP16 Spe Pst 2
| 36.9 || 1.73
|-
!  RXRLc Bam Xho 1
| 7.7 || 2.51
|-
!  RXRLc Bam Xho 2
| 4.1 || 1.93
|-
!  35s min anneal 1/100 from 8/2
| 3.6 || 1.31
|-
!  RXRLc Xba Pst
| 3.6 || 1.31
|-
|}




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__NOTOC__
__NOTOC__

Revision as of 15:19, 7 September 2010

iGEM iGarden <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Team Allergy

  1. Redigest V9/V10 (three digests of each backbone to use more DNA in ligations)
  2. Grew up V9/V10+ ihpRNA colonies
  3. Re pcrd amiRNA and digested to ligate into V9/V10
  4. Catalog parts to be ready for the registry

Team Fence

Gel Extracted Friday's Digests

Nanodrop of Friday's Digest Gel Extractions

Plasmid Quantity (ng/μL) 260/280
Barstar Eco Pst 1 .9 -.57
Barstar Eco Pst 2 -.4 .14
RXRLc Xba Pst .3 -.19
Barnase Eco Pst 1 20.5 1.64
Barnase Eco Pst 2 14.4 1.83
5xGalUAS Spe Pst 1 10.7 1.87
5xGalUAS Spe Pst 2 16.4 1.90
pENTcup Spe Pst 1 16.3 1.88
pENTcup Spe Pst 2 14.7 2.09
PhyB Bam Nco 1 1.7 -1.10
PhyB Bam Nco 2 -.6 .24
V26 Eco Pst 1 2.4 1.6
V26 Eco Pst 2 1.3 1.05
V26 Eco Pst 3 1.4 3.01
V24 Eco Pst 1 8.3 3.6
V24 Eco Pst 2 5.5 1.64
VP16 Eco Pst 1 3.4 1.6
VP16 Spe Pst 1 9.4 1.92
VP16 Spe Pst 2 36.9 1.73
RXRLc Bam Xho 1 7.7 2.51
RXRLc Bam Xho 2 4.1 1.93
35s min anneal 1/100 from 8/2 3.6 1.31
RXRLc Xba Pst 3.6 1.31


Redo of some digests with no insert where there should have been an insert

  • ladder
  • pACT2 (backbone)
  • EcRGal (insert)- STILL NO INSERT
  • RxRLC (insert) Xba and Pst
  • RxRLC (insert) Bam and Xho
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