IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/10

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2010/08/10 Entry for IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010)
Line 5: Line 5:
|-
|-
| colspan="2"|
| colspan="2"|
-
==Entry title==
+
==Team Allergy==
-
* Insert your content here.
+
'''ihpRNA'''
-
 
+
*Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ''ger ihprna constructs''
 +
**growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
 +
**concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
 +
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
 +
[[Image: |240 px|Diagnostic Digest Ger ihpRNA into V10]]
 +
*Electroporation to transform into agrobacterium
 +
'''amiRNA'''
 +
*Ligated into V9/V10 and transformed
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->

Revision as of 13:04, 10 August 2010

iGEM iGarden Main project page
Previous entry      Next entry

Team Allergy

ihpRNA

  • Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ger ihprna constructs
    • growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
    • concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
  • Diagnostic Digest (Should see ~ 7 kb band and 840 band)

[[Image: |240 px|Diagnostic Digest Ger ihpRNA into V10]]

  • Electroporation to transform into agrobacterium

amiRNA

  • Ligated into V9/V10 and transformed


Personal tools