IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/10

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(→Team Allergy)
Line 11: Line 11:
**concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
**concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
-
[[Image: |240 px|Diagnostic Digest Ger ihpRNA into V10]]
+
[[Image: agroddigest.jpg|240 px|Diagnostic Digest Ger ihpRNA into V10]]
*Electroporation to transform into agrobacterium
*Electroporation to transform into agrobacterium

Revision as of 13:29, 10 August 2010

iGEM iGarden Main project page
Previous entry      Next entry

Team Allergy

ihpRNA

  • Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ger ihprna constructs
    • growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
    • concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
  • Diagnostic Digest (Should see ~ 7 kb band and 840 band)

Diagnostic Digest Ger ihpRNA into V10

  • Electroporation to transform into agrobacterium

amiRNA

  • Ligated into V9/V10 and transformed


Retrieved from "http://www.openwetware.org/wiki/IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010/2010/08/10"
Personal tools