IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/10

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(Team Allergy)
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**concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
**concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
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[[Image: |240 px|Diagnostic Digest Ger ihpRNA into V10]]
+
[[Image: agroddigest.jpg|240 px|Diagnostic Digest Ger ihpRNA into V10]]
*Electroporation to transform into agrobacterium
*Electroporation to transform into agrobacterium

Revision as of 13:29, 10 August 2010

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Team Allergy

ihpRNA

  • Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ger ihprna constructs
    • growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
    • concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
  • Diagnostic Digest (Should see ~ 7 kb band and 840 band)

Diagnostic Digest Ger ihpRNA into V10

  • Electroporation to transform into agrobacterium

amiRNA

  • Ligated into V9/V10 and transformed


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