IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/10

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(Team Allergy)
(Team Allergy)
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*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
*Diagnostic Digest (Should see ~ 7 kb band and 840 band)
[[Image: agroddigest.jpg|240 px|Diagnostic Digest Ger ihpRNA into V10]]
[[Image: agroddigest.jpg|240 px|Diagnostic Digest Ger ihpRNA into V10]]
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*Electroporation to transform into agrobacterium
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*Did not see exepcted bands (waiting on cultures grown up today to miniprep and hopefully transform into agrobacterium tommorow)
'''amiRNA'''
'''amiRNA'''

Revision as of 13:50, 10 August 2010

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Team Allergy

ihpRNA

  • Only 2 colonies grew up (V10 9 (37c1) and V10 12 B(38 c2)) ger ihprna constructs
    • growing up colonies again in 5 mL cultures w/less antibiotic (50 ng/uL of kan)
    • concentrations: V10_9: 21.4 ng/uL V10_12B: 16.9 ng/uL
  • Diagnostic Digest (Should see ~ 7 kb band and 840 band)

Diagnostic Digest Ger ihpRNA into V10

  • Did not see exepcted bands (waiting on cultures grown up today to miniprep and hopefully transform into agrobacterium tommorow)

amiRNA

  • Ligated into V9/V10 and transformed


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