IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/11: Difference between revisions

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**Concentrations (20-60 ng/uL range)
**Concentrations (20-60 ng/uL range)
*Diagnostic Digest (should see ~ 7 kb and 840 bp)
*Diagnostic Digest (should see ~ 7 kb and 840 bp)
[[Image: |240px]]
[[Image: V910ddigestconfirm.jpg|240px]]


Ladder, V9 5 (Bet2), V91(LTP), V99 (Ger), V99B (Ger), V1011 (Ger), V107b(Ger), V1012 (Ger), V109B (Ger)
Ladder, V9 5 (Bet2), V91(LTP), V99 (Ger), V99B (Ger), V1011 (Ger), V107b(Ger), V1012 (Ger), V109B (Ger)
V10 7B (Ger worked)


==Team Fence==
==Team Fence==


===Digests 8/10===
===colonies from yesterday's transformations/ligations===
 
[[Image: 810 colonies 1.jpg]]
[[Image: 810 colonies 2.jpg]]
 
basically nothing on either
 
[[Image: 810 colonies 3.jpg]]
[[Image: 810 colonies 4.jpg]]
 
nothing on either
 
 
[[Image: 810 colonies 6.jpg]]




[[Image: 810 colonies last.jpg]]


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Revision as of 12:23, 18 August 2010

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Team Allergy

  • Minipreps of (V95, V91, V99, V99b, V1011, V107B, V1012, V109b)
    • Concentrations (20-60 ng/uL range)
  • Diagnostic Digest (should see ~ 7 kb and 840 bp)

Ladder, V9 5 (Bet2), V91(LTP), V99 (Ger), V99B (Ger), V1011 (Ger), V107b(Ger), V1012 (Ger), V109B (Ger)

V10 7B (Ger worked)

Team Fence

colonies from yesterday's transformations/ligations

basically nothing on either

nothing on either