IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12: Difference between revisions
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Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2) | Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2) | ||
*Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10) | *Digested V9/V10 backbone for ligations w/ Eco & Pst (redoing ihpRNA ligations into V9/V10) | ||
[[Image: |240px|backbone]] | [[Image: 2010-08-12_13hr_10min.jpg|240px|backbone]] | ||
'''amiRNA''' | '''amiRNA''' | ||
Revision as of 12:32, 12 August 2010
 iGEM iGarden
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Team AllergyihpRNA
ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2)
amiRNA
Team Fence
This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation.
Transformations from Yesterday's Ligations
Colonies beyond control on RXRLc VP16, Barstar V26, and EcR-Gal-PhyB.
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