IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12: Difference between revisions
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*Minipreps of more V9/V10 colonies | *Minipreps of more V9/V10 colonies | ||
*Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp) | *Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp) | ||
[[Image: |240px|diagnostic digest of ihpRNA in agro vectors]] | [[Image: Faildigest.jpg|240px|diagnostic digest of ihpRNA in agro vectors]] | ||
ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b | ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b | ||
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Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2) | Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2) | ||
*Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10) | *Digested V9/V10 backbone for ligations w/ Eco & Pst (redoing ihpRNA ligations into V9/V10) | ||
[[Image: |240px|backbone]] | [[Image: 2010-08-12_13hr_10min.jpg|240px|backbone]] | ||
'''amiRNA''' | '''amiRNA''' | ||
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==Team Fence== | ==Team Fence== | ||
[[Image: arp2coloniesligb21.jpg]] | [[Image: arp2coloniesligb21.jpg||350px]] | ||
This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation. | This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation. | ||
[[Image: 812 5xgaluas.jpg]] | |||
transformation from karmella's stock | |||
===Transformations from | |||
===Transformations from Yesterday's Ligations=== | |||
[[Image:RXRLc_VP16_8-12.jpg||350px]] | [[Image:RXRLc_VP16_8-12.jpg||350px]] | ||
[[Image:Barstar_pCola_lig_8-12.jpg||350px]] | [[Image:Barstar_pCola_lig_8-12.jpg||350px]] | ||
[[Image:EcR-Gal-PhyB_lig_8-12.jpg||350px]] | [[Image:EcR-Gal-PhyB_lig_8-12.jpg||350px]] | ||
[[Image:Untrans_cont_8-12.jpg]] | [[Image:Untrans_cont_8-12.jpg||350px]] | ||
[[Image:Gal4_EcR_lig_8-12.jpg||350px]] | |||
[[Image:Barstar_pDUET_lig_8-12.jpg||350px]] | |||
[[Image:Barstar_pCOLA_lig_from_8-10.jpg||350px]] | |||
[[Image:V26_cont_8-12.jpg||350px]] | |||
[[Image:VP16_cont_lig_8-12.jpg||350px]] | |||
[[Image:ACC_-_Barnase_lig_8-12.jpg||350px]] | |||
[[Image:Barnase_cont_lig_8-12.jpg||350px]] | |||
[[Image:Barnase_pDUET_8-12.jpg||350px]] | |||
[[Image:RXRLc_VP16_lig_8-12.jpg||350px]] | |||
[[Image:PhyB_cont_lig_8-12.jpg||350px]] | |||
[[Image:V24_Control_lig_8-12.jpg||350px]] | |||
Colonies beyond control on RXRLc VP16, Barstar V26, and EcR-Gal-PhyB. | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
Revision as of 12:07, 18 August 2010
 iGEM iGarden
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Team AllergyihpRNA
ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2)
amiRNA
Team Fence
This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation.
transformation from karmella's stock
Transformations from Yesterday's Ligations
Colonies beyond control on RXRLc VP16, Barstar V26, and EcR-Gal-PhyB.
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