IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12
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| + | ==Team Allergy== | ||
| + | '''ihpRNA''' | ||
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| + | *Minipreps of more V9/V10 colonies | ||
| + | *Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp) | ||
| + | [[Image: |240px|diagnostic digest of ihpRNA in agro vectors]] | ||
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| + | ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b | ||
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| + | Lanes 1&2: LTP (11c1); Lanes 3&4 Bet2 (25c1)); Lane 5 Bet2 (25c2); Lane 6: Bet2 (28c1); Lanes 7 and 8 Ger (36c1); Lanes 9&10 Ger (36c2) | ||
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| + | *Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10) | ||
| + | [[Image: |240px|backbone]] | ||
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| + | '''amiRNA''' | ||
| + | |||
| + | *Ligated into V9/V10 and transformed (mutagenesis sites confirmed through pcr using 20 bp sepecific primers) | ||
| + | **still need to be sequenced | ||
| + | |||
| + | |||
| + | [[Image: | ||
==Team Fence== | ==Team Fence== | ||
Revision as of 15:11, 12 August 2010
 iGEM iGarden
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Team AllergyihpRNA
[[Image: |240px|diagnostic digest of ihpRNA in agro vectors]] ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b Lanes 1&2: LTP (11c1); Lanes 3&4 Bet2 (25c1)); Lane 5 Bet2 (25c2); Lane 6: Bet2 (28c1); Lanes 7 and 8 Ger (36c1); Lanes 9&10 Ger (36c2)
[[Image: |240px|backbone]] amiRNA
Team Fence
This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation.
Transformations from yesterday
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