IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/12

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(Team Allergy)
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ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b
ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b
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Lanes 1&2: LTP (11c1); Lanes 3&4 Bet2 (25c1)); Lane 5 Bet2 (25c2); Lane 6: Bet2 (28c1); Lanes 7 and 8 Ger (36c1); Lanes 9&10 Ger (36c2)
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Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2)
*Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10)
*Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10)
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[[Image:
[[Image:
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==Team Fence==
==Team Fence==

Revision as of 15:15, 12 August 2010

iGEM iGarden Main project page
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Team Allergy

ihpRNA

  • Minipreps of more V9/V10 colonies
  • Diagnostic Digest of minipreps w/ Eco and Pst (Should see ~840 and 7000 bp)

[[Image: |240px|diagnostic digest of ihpRNA in agro vectors]]

ladder, V92, V92b, V94, V94b, V95, V96b, V97, V97b, V98, V98b

Lanes 2&3: LTP (11c1); Lanes 4&5: Bet2 (25c1); Lane 6: Bet2 (25c2); Lane 7: Bet2 (28c1); Lanes 8&9: Ger (36c1); Lanes 10&11 Ger (36c2)

  • Digested V9/V10 backbone for ligations (redoing ihpRNA ligations into V9/V10)

[[Image: |240px|backbone]]

amiRNA

  • Ligated into V9/V10 and transformed (mutagenesis sites confirmed through pcr using 20 bp sepecific primers)
    • still need to be sequenced


[[Image:

Team Fence

Image: arp2coloniesligb21.jpg

This picture is not the best picture but we did find colonies on the plate with the arp2/b21 transformation.


Transformations from yesterday

Image:Untrans_cont_8-12.jpg


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