IGEM:Harvard/2010/Brainstorming/food: Difference between revisions
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[http://www.bio.davidson.edu/people/kabernd/seminar/2002/method/dsmeth/EJB%20Figure.jpg Agrobacterium infection diagram] | [http://www.bio.davidson.edu/people/kabernd/seminar/2002/method/dsmeth/EJB%20Figure.jpg Agrobacterium infection diagram] | ||
3. Antunes, et. al. [http://www3.interscience.wiley.com.ezp-prod1.hul.harvard.edu/cgi-bin/fulltext/118585509/HTMLSTART] | |||
===protocols=== | ===protocols=== |
Revision as of 11:27, 3 May 2010
Strains, Plasmids, and Trasformation
references
1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.
2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Agrobacterium infection diagram
3. Antunes, et. al. [2]
protocols
- Book: Agrobacterium Protocols
RNAi
- Chuang CF and Meyerowitz EM. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90. DOI:10.1073/pnas.060034297 |
Markers and Kill Switches
Dual selection system (aka killswitch) ideas
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
Downstream effectors
allergens
- Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci. 2008 Jun;13(6):257-60. DOI:10.1016/j.tplants.2008.04.004 |
- Dodo H, Konan K, and Viquez O. A genetic engineering strategy to eliminate peanut allergy. Curr Allergy Asthma Rep. 2005 Jan;5(1):67-73. DOI:10.1007/s11882-005-0058-0 |
flavors
- [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde
- Sinha AK, Sharma UK, and Sharma N. A comprehensive review on vanilla flavor: extraction, isolation and quantification of vanillin and others constituents. Int J Food Sci Nutr. 2008 Jun;59(4):299-326. DOI:10.1080/09687630701539350 |
http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#