IGEM:Harvard/2010/Brainstorming/food

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(New page: Dual selection system (aka killswitch) ideas: - use selectable markers such as URA3 to control whether an integration plasmid is replicated or not -- cells with the URA3 gene can grow on m...)
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Dual selection system (aka killswitch) ideas:
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Dual selection system (aka killswitch) ideas:<br>
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- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
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- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not<br>
-
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
+
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)<br>
-
-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
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-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br>

Revision as of 13:20, 28 April 2010

Dual selection system (aka killswitch) ideas:
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA

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