IGEM:Harvard/2010/Brainstorming/food
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(New page: Dual selection system (aka killswitch) ideas: - use selectable markers such as URA3 to control whether an integration plasmid is replicated or not -- cells with the URA3 gene can grow on m...) |
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| - | Dual selection system (aka killswitch) ideas: | + | Dual selection system (aka killswitch) ideas:<br> |
| - | - use selectable markers such as URA3 to control whether an integration plasmid is replicated or not | + | - use selectable markers such as URA3 to control whether an integration plasmid is replicated or not<br> |
| - | -- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA) | + | -- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)<br> |
| - | -- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA | + | -- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br> |
Revision as of 14:20, 28 April 2010
Dual selection system (aka killswitch) ideas:
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
-- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
-- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
