IGEM:Harvard/2010/Brainstorming/food

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(RNAi)
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*[http://partsregistry.org/IGEM:MIT/2006/AL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM  2006]
*[http://partsregistry.org/IGEM:MIT/2006/AL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM  2006]
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====miraculin====
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*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4HYD5PG-5&_user=10&_coverDate=01%2F23%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=3a7e5c42ef7261e48ea4e9b523d8312a Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce]
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*[http://jb.oxfordjournals.org.ezp-prod1.hul.harvard.edu/cgi/reprint/145/4/445 Functional Expression of Miraculin, a Taste-Modifying Protein in Escherichia Coli]
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Revision as of 13:56, 3 May 2010

Contents

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium infection diagram

3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity

protocols

RNAi

  1. Chuang CF and Meyerowitz EM. . pmid:10781109. PubMed HubMed [1]


Markers and Kill Switches

Dual selection system (aka killswitch) ideas

  • use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  • cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  • cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA


Downstream effectors

allergens

  1. Singh MB and Bhalla PL. . pmid:18467156. PubMed HubMed [1]
  2. Dodo H, Konan K, and Viquez O. . pmid:15659267. PubMed HubMed [2]
All Medline abstracts: PubMed HubMed

flavors

  • [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde

miraculin

  1. Theerasilp S and Kurihara Y. . pmid:3403544. PubMed HubMed [1]
  1. Sinha AK, Sharma UK, and Sharma N. . pmid:17886091. PubMed HubMed [2]

http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#

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