IGEM:Harvard/2010/Brainstorming/food

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*cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br>
*cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br>
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===De-Greening===
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*this paper has some parts that could be useful as kill-switches
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*[http://www.ncbi.nlm.nih.gov/pubmed/17309732 A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.]
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*steroid-inducible chlorophyll knockdown
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==Downstream effectors==
==Downstream effectors==
===allergens===
===allergens===

Revision as of 20:19, 4 May 2010

Contents

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium infection diagram

3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity

protocols

RNAi

  1. Chuang CF and Meyerowitz EM. . pmid:10781109. PubMed HubMed [1]


Markers and Kill Switches

Dual selection system (aka killswitch) ideas

  • use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  • cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  • cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA




De-Greening

Downstream effectors

allergens

  1. Singh MB and Bhalla PL. . pmid:18467156. PubMed HubMed [1]
  2. Dodo H, Konan K, and Viquez O. . pmid:15659267. PubMed HubMed [2]
All Medline abstracts: PubMed HubMed

flavors

  • [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde

VANILLA: http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#

miraculin

  1. Theerasilp S and Kurihara Y. . pmid:3403544. PubMed HubMed [1]
  1. Sinha AK, Sharma UK, and Sharma N. . pmid:17886091. PubMed HubMed [2]


caffeine etc.

  1. Ashihara H, Sano H, and Crozier A. . pmid:18068204. PubMed HubMed [1]
  1. http://www.plantcell.org/cgi/content/abstract/6/5/723 [2]
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