IGEM:Harvard/2010/Brainstorming/food
From OpenWetWare
(Difference between revisions)
| Line 25: | Line 25: | ||
*cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br> | *cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br> | ||
<br> | <br> | ||
| - | + | ||
| - | + | ||
===De-Greening=== | ===De-Greening=== | ||
*this paper has some parts that could be useful as kill-switches | *this paper has some parts that could be useful as kill-switches | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/17309732 A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.] | *[http://www.ncbi.nlm.nih.gov/pubmed/17309732 A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.] | ||
*steroid-inducible chlorophyll knockdown | *steroid-inducible chlorophyll knockdown | ||
| - | + | <br> | |
| + | <br> | ||
==Downstream effectors== | ==Downstream effectors== | ||
===allergens=== | ===allergens=== | ||
Revision as of 20:19, 4 May 2010
Contents |
Strains, Plasmids, and Trasformation
references
1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.
2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
Agrobacterium infection diagram
3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity
protocols
- Book: Agrobacterium Protocols
RNAi
Error fetching PMID 10781109:
- Error fetching PMID 10781109:
Markers and Kill Switches
Dual selection system (aka killswitch) ideas
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
De-Greening
- this paper has some parts that could be useful as kill-switches
- A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.
- steroid-inducible chlorophyll knockdown
Downstream effectors
allergens
Error fetching PMID 18467156:
Error fetching PMID 15659267:
Error fetching PMID 15659267:
- Error fetching PMID 18467156:
- Error fetching PMID 15659267:
flavors
- [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde
VANILLA: http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#
miraculin
Error fetching PMID 3403544:
- Error fetching PMID 3403544:
Error fetching PMID 17886091:
- Error fetching PMID 17886091:
caffeine etc.
Error fetching PMID 18068204:
- Error fetching PMID 18068204:
