IGEM:Harvard/2010/Brainstorming/food

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*cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br>
*cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA<br>
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===De-Greening===
===De-Greening===
*this paper has some parts that could be useful as kill-switches
*this paper has some parts that could be useful as kill-switches
*[http://www.ncbi.nlm.nih.gov/pubmed/17309732 A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.]
*[http://www.ncbi.nlm.nih.gov/pubmed/17309732 A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity.]
*steroid-inducible chlorophyll knockdown
*steroid-inducible chlorophyll knockdown
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==Downstream effectors==
==Downstream effectors==
===allergens===
===allergens===

Revision as of 19:19, 4 May 2010

Contents

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium infection diagram

3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity

protocols

RNAi

Error fetching PMID 10781109:
  1. Error fetching PMID 10781109: [1]


Markers and Kill Switches

Dual selection system (aka killswitch) ideas

  • use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  • cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  • cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA


De-Greening



Downstream effectors

allergens

Error fetching PMID 18467156:
Error fetching PMID 15659267:
  1. Error fetching PMID 18467156: [1]
  2. Error fetching PMID 15659267: [2]
All Medline abstracts: PubMed HubMed

flavors

  • [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde

VANILLA: http://www.3dchem.com/moremolecules.asp?ID=307&othername=Vanilla#

miraculin

Error fetching PMID 3403544:
  1. Error fetching PMID 3403544: [1]
Error fetching PMID 17886091:
  1. Error fetching PMID 17886091: [2]


caffeine etc.

Error fetching PMID 18068204:
  1. Error fetching PMID 18068204: [1]
  1. http://www.plantcell.org/cgi/content/abstract/6/5/723 [2]
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