IGEM:Harvard/2010/Brainstorming/food

Strains, Plasmids, and Trasformation

references

1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

protocols

• Book: Agrobacterium Protocols

RNAi

1. Chuang CF and Meyerowitz EM. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90. DOI:10.1073/pnas.060034297 |

Markers and Kill Switches

Dual selection system (aka killswitch) ideas

• use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  
• cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  
• cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
  

Downstream effectors

allergens

1. Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci. 2008 Jun;13(6):257-60. DOI:10.1016/j.tplants.2008.04.004 |
2. Dodo H, Konan K, and Viquez O. A genetic engineering strategy to eliminate peanut allergy. Curr Allergy Asthma Rep. 2005 Jan;5(1):67-73. DOI:10.1007/s11882-005-0058-0 |

flavors

• Growth dependent banana odor generator, MIT iGEM 2006

1. Theerasilp S and Kurihara Y. Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. J Biol Chem. 1988 Aug 15;263(23):11536-9.