IGEM:Harvard/2010/Brainstorming/food
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Strains, Plasmids, and Trasformation
references
1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.
2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool
protocols
- Book: Agrobacterium Protocols
RNAi
- Chuang CF and Meyerowitz EM. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90. DOI:10.1073/pnas.060034297 |
Markers and Kill Switches
Dual selection system (aka killswitch) ideas
- use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
- cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
- cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA
Downstream effectors
allergens
- Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci. 2008 Jun;13(6):257-60. DOI:10.1016/j.tplants.2008.04.004 |
- Dodo H, Konan K, and Viquez O. A genetic engineering strategy to eliminate peanut allergy. Curr Allergy Asthma Rep. 2005 Jan;5(1):67-73. DOI:10.1007/s11882-005-0058-0 |