IGEM:Harvard/2010/General Protocols: Difference between revisions
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*[[Silver: Bacterial Transformation | Bacterial Transformation]] | *[[Silver: Bacterial Transformation | Bacterial Transformation]] | ||
*[[Silver: Plasmid Verification | Plasmid Verification]] | *[[Silver: Plasmid Verification | Plasmid Verification]] | ||
*[http://labs.fhcrc.org/fero/Protocols/RNeasy_Mini_Handbook.pdf Qiagen RNeasy Plant Mini Kit] | |||
*[http://cshprotocols.cshlp.org/cgi/reprint/2007/4/pdb.kit23.pdf iScript cDNA Synthesis] | |||
=Microbiology= | =Microbiology= | ||
Revision as of 08:35, 15 June 2010
Synthetic Biology
- How Do BioBricks Work?
- Strategy for Making Parts
- PCR
- Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- DNA Gel Electrophoresis and Fragment Purification
- Measuring DNA Concentration Using a Nanodrop Spectrophotometer
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Qiagen RNeasy Plant Mini Kit
- iScript cDNA Synthesis
