IGEM:Harvard/2010/General Protocols

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(Growing a Bacterial E. coli Culture)
Current revision (14:54, 1 July 2010) (view source)
(Microbiology)
 
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=Growing a Bacterial E. coli Culture=
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=Synthetic Biology=
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E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is
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*[[IGEM:Harvard/2010/General Protocols/BioBricks | How Do BioBricks Work?]]
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*[[Silver: BB Strategy | Strategy for Making Parts]]
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*[[Silver: PCR | PCR]]
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*[[Silver: Oligonucleotide Inserts | Oligonucleotide Inserts]]
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*[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]]
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*[[Silver: Restriction Digest | Restriction Digest]]
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*[[IGEM:Harvard/2010/General Protocols/DNApurification | DNA Gel Electrophoresis and Fragment Purification]]
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*[[IGEM:Harvard/2010/General Protocols/Nanodrop | Measuring DNA Concentration Using a Nanodrop Spectrophotometer]]
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*[[Silver: Ligation | Ligation]]
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*[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]]
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*[[Silver: Bacterial Transformation | Bacterial Transformation]]
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*[[Silver: Plasmid Verification | Plasmid Verification]]
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*[http://labs.fhcrc.org/fero/Protocols/RNeasy_Mini_Handbook.pdf Qiagen RNeasy Plant Mini Kit]
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*[http://cshprotocols.cshlp.org/cgi/reprint/2007/4/pdb.kit23.pdf iScript cDNA Synthesis]
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=Plasmid Minipreps: Qiagen Miniprep Kit=
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=Microbiology=
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=DNA Digests=
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*[[IGEM:Harvard/2010/General Protocols/EColi | Growing E. coli]]
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=DNA Gel Electrophoresis and Fragment Purification=
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*[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]]
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=Measuring DNA Concentration using a Nanodrop Spectrophotometer=
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*[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]]
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=Ligation Reaction=
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*[[IGEM:Harvard/2010/General Protocols/Agrobacterium Electroporation | Electroporating Agrobacterium]]
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=Transformation of E. coli=
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=Confirming the Assembly=
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=Plants=
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=Growing Arabidopsis=
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*[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]]
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=Growing an Agrobacterium Culture=
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*[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]]
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=Arabidopsis transformation via Flower Dipping=
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Current revision

Synthetic Biology

Microbiology

Plants

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