IGEM:Harvard/2010/General Protocols: Difference between revisions

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=Growing a Bacterial E. coli Culture=
=Synthetic Biology=
E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is
*[[IGEM:Harvard/2010/General Protocols/BioBricks | How Do BioBricks Work?]]
*[[Silver: BB Strategy | Strategy for Making Parts]]
*[[Silver: PCR | PCR]]
*[[Silver: Oligonucleotide Inserts | Oligonucleotide Inserts]]
*[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]]
*[[Silver: Restriction Digest | Restriction Digest]]
*[[IGEM:Harvard/2010/General Protocols/DNApurification | DNA Gel Electrophoresis and Fragment Purification]]
*[[IGEM:Harvard/2010/General Protocols/Nanodrop | Measuring DNA Concentration Using a Nanodrop Spectrophotometer]]
*[[Silver: Ligation | Ligation]]
*[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]]
*[[Silver: Bacterial Transformation | Bacterial Transformation]]
*[[Silver: Plasmid Verification | Plasmid Verification]]
*[http://labs.fhcrc.org/fero/Protocols/RNeasy_Mini_Handbook.pdf Qiagen RNeasy Plant Mini Kit]
*[http://cshprotocols.cshlp.org/cgi/reprint/2007/4/pdb.kit23.pdf iScript cDNA Synthesis]


=Plasmid Minipreps: Qiagen Miniprep Kit=
=Microbiology=
To extract plasmid DNA from bacteria, perform a miniprep using the Qiagen miniprep protocol.
*[[IGEM:Harvard/2010/General Protocols/EColi | Growing E. coli]]
*[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]]
*[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]]
*[[IGEM:Harvard/2010/General Protocols/Agrobacterium Electroporation | Electroporating Agrobacterium]]


=DNA Digests=
=Plants=
=DNA Gel Electrophoresis and Fragment Purification=
*[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]]
=Measuring DNA Concentration using a Nanodrop Spectrophotometer=
*[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]]
=Ligation Reaction=
=Transformation of E. coli=
=Confirming the Assembly=
=Growing Arabidopsis=
=Growing an Agrobacterium Culture=
=Arabidopsis transformation via Flower Dipping=

Latest revision as of 11:54, 1 July 2010

Synthetic Biology

Microbiology

Plants

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