IGEM:Harvard/2010/General Protocols
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< IGEM:Harvard | 2010(Difference between revisions)
Current revision (14:54, 1 July 2010) (view source) (→Microbiology) |
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*[[Silver: Bacterial Transformation | Bacterial Transformation]] | *[[Silver: Bacterial Transformation | Bacterial Transformation]] | ||
*[[Silver: Plasmid Verification | Plasmid Verification]] | *[[Silver: Plasmid Verification | Plasmid Verification]] | ||
| + | *[http://labs.fhcrc.org/fero/Protocols/RNeasy_Mini_Handbook.pdf Qiagen RNeasy Plant Mini Kit] | ||
| + | *[http://cshprotocols.cshlp.org/cgi/reprint/2007/4/pdb.kit23.pdf iScript cDNA Synthesis] | ||
=Microbiology= | =Microbiology= | ||
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*[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]] | *[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]] | ||
*[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]] | *[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]] | ||
| + | *[[IGEM:Harvard/2010/General Protocols/Agrobacterium Electroporation | Electroporating Agrobacterium]] | ||
=Plants= | =Plants= | ||
*[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]] | *[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]] | ||
*[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]] | *[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]] | ||
Current revision
Synthetic Biology
- How Do BioBricks Work?
- Strategy for Making Parts
- PCR
- Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- DNA Gel Electrophoresis and Fragment Purification
- Measuring DNA Concentration Using a Nanodrop Spectrophotometer
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Qiagen RNeasy Plant Mini Kit
- iScript cDNA Synthesis
Microbiology
- Growing E. coli
- Growing Agrobacterium
- Making Electro-Competent Agrobacterium
- Electroporating Agrobacterium
