IGEM:Harvard/2010/General Protocols: Difference between revisions
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*[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]] | *[[IGEM:Harvard/2010/General Protocols/Agrobacterium | Growing Agrobacterium]] | ||
*[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]] | *[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Making Electro-Competent Agrobacterium]] | ||
*[[IGEM:Harvard/2010/General Protocols/CompetentAgrobacterium | Agrobacterium Electroporation]] | |||
=Plants= | =Plants= | ||
*[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]] | *[[IGEM:Harvard/2010/General Protocols/Arabidopsis | Growing Arabidopsis]] | ||
*[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]] | *[[IGEM:Harvard/2010/General Protocols/FlowerDipping | Arabidopsis transformation via Flower Dipping]] | ||
Revision as of 11:52, 1 July 2010
Synthetic Biology
- How Do BioBricks Work?
- Strategy for Making Parts
- PCR
- Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- DNA Gel Electrophoresis and Fragment Purification
- Measuring DNA Concentration Using a Nanodrop Spectrophotometer
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Qiagen RNeasy Plant Mini Kit
- iScript cDNA Synthesis
Microbiology
- Growing E. coli
- Growing Agrobacterium
- Making Electro-Competent Agrobacterium
- Agrobacterium Electroporation
