IGEM:Harvard/2010/General Protocols

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(Growing a Bacterial E. coli Culture)
(Plasmid Minipreps: Qiagen Miniprep Kit)
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=Plasmid Minipreps: Qiagen Miniprep Kit=
=Plasmid Minipreps: Qiagen Miniprep Kit=
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To extract plasmid DNA from bacteria, perform a miniprep using the Qiagen miniprep protocol.
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=DNA Digests=
=DNA Digests=
=DNA Gel Electrophoresis and Fragment Purification=
=DNA Gel Electrophoresis and Fragment Purification=

Revision as of 13:37, 17 May 2010

Contents

Growing a Bacterial E. coli Culture

E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is

Plasmid Minipreps: Qiagen Miniprep Kit

To extract plasmid DNA from bacteria, perform a miniprep using the Qiagen miniprep protocol.

DNA Digests

DNA Gel Electrophoresis and Fragment Purification

Measuring DNA Concentration using a Nanodrop Spectrophotometer

Ligation Reaction

Transformation of E. coli

Confirming the Assembly

Growing Arabidopsis

Growing an Agrobacterium Culture

Arabidopsis transformation via Flower Dipping

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