IGEM:Harvard/2010/General Protocols

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Contents

Growing a Bacterial E. coli Culture

E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is

Plasmid Minipreps: Qiagen Miniprep Kit

DNA Digests

DNA Gel Electrophoresis and Fragment Purification

Measuring DNA Concentration using a Nanodrop Spectrophotometer

Ligation Reaction

Transformation of E. coli

Confirming the Assembly

Growing Arabidopsis

Growing an Agrobacterium Culture

Arabidopsis transformation via Flower Dipping

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