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Growing a Bacterial E. coli Culture
E. coli is a typical bacteria used for molecular cloning. Long-term storage of BioBrick plasmids is
Plasmid Minipreps: Qiagen Miniprep Kit
To extract plasmid DNA from bacteria, perform a miniprep using the Qiagen miniprep protocol.
DNA Digests
DNA Gel Electrophoresis and Fragment Purification
Measuring DNA Concentration using a Nanodrop Spectrophotometer
Ligation Reaction
Transformation of E. coli
Confirming the Assembly
Growing Arabidopsis
Growing an Agrobacterium Culture
Arabidopsis transformation via Flower Dipping
