IGEM:Harvard/2010/General Protocols/Agrobacterium Electroporation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search

Devin R. Burrill (Talk | contribs)
(New page: Safety precautions: *Never change any other settings than stated while unit is charging (this may damage both the electroporator and powerssupply) *Keep unit away from water in a dry area...)
Next diff →

Revision as of 13:58, 1 July 2010

Safety precautions:

  • Never change any other settings than stated while unit is charging (this may damage both the electroporator and powerssupply)
  • Keep unit away from water in a dry area and away from flammable materials
  • Never short circuit terminals
  • Whilst delivering pulse, keep hands away from chamber and cuvette. The result may otherwise be shocking

DNA preparations:

  • DNA for electroporation must be free of salt, RNA, or protein. To accomplish this, use Millipore Nitrocellulose Membrane Filters (Cat. No. VSWPO2500) and follow manufacturer's instructions

Preparing the electroporator:

  • Use 2mm cuvettes from either Invitrogen
Personal tools