IGEM:Harvard/2010/General Protocols/Agrobacterium Electroporation: Difference between revisions
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(New page: Safety precautions: *Never change any other settings than stated while unit is charging (this may damage both the electroporator and powerssupply) *Keep unit away from water in a dry area...) |
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Revision as of 11:58, 1 July 2010
Safety precautions:
- Never change any other settings than stated while unit is charging (this may damage both the electroporator and powerssupply)
- Keep unit away from water in a dry area and away from flammable materials
- Never short circuit terminals
- Whilst delivering pulse, keep hands away from chamber and cuvette. The result may otherwise be shocking
DNA preparations:
- DNA for electroporation must be free of salt, RNA, or protein. To accomplish this, use Millipore Nitrocellulose Membrane Filters (Cat. No. VSWPO2500) and follow manufacturer's instructions
Preparing the electroporator:
- Use 2mm cuvettes from either Invitrogen
