IGEM:Harvard/2010/Team Allergy: Difference between revisions
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We are designing shRNA constructs against common allergens in fruits and vegetables. | We are designing shRNA constructs against common allergens in fruits and vegetables. | ||
==Bibliography== | ==Bibliography== | ||
<biblio> | |||
#1 pmid = 11576441 | |||
#2 pmid = 17784907 | |||
</biblio> | |||
==Primers and Sequences== | ==Primers and Sequences== | ||
Revision as of 10:34, 14 June 2010
Description
We are designing shRNA constructs against common allergens in fruits and vegetables.
Bibliography
-
pmid = 11576441
-
pmid = 17784907
Primers and Sequences
| name | GenBank Accession | forward sense primer | reverse sense primer | forward antisense primer | reverse antisense primer |
|---|---|---|---|---|---|
| pdk intron | AJ311872 | CCTTGAATTCGCGGCCGCATCTAGA tcttttttccttttagtata | AAGGCTGCAGCGGCCGCTACTAGT CAATCCAAATGTAAGATCAA | ||
| A. thaliana LTP1 | ATHSEQB | CCTTGAATTCGCGGCCGCATCTAGA ATGGCTGGAGTGATGAAGTT | AAGGCTGCAGCGGCCGCTACTAGT CAGCTGCACGGCCAGCGTTG | AAGGCTGCAGCGGCCGCTACTAGT ATGGCTGGAGTGATGAAGTT | CCTTGAATTCGCGGCCGCATCTAGA CAGCTGCACGGCCAGCGTTG |
| Fra a 1A | DQ385511 | CCTTGAATTCGCGGCCGCATCTAGA ATGGGTGTTTACACTTATGA | AAGGCTGCAGCGGCCGCTACTAGT GTAGTCGATCTTCTCGATGT | AAGGCTGCAGCGGCCGCTACTAGT ATGGGTGTTTACACTTATGA | CCTTGAATTCGCGGCCGCATCTAGA GTAGTCGATCTTCTCGATGT |
| L. sativa LTP 1.1 | EF101532.1 | CCTTGAATTCGCGGCCGCATCTAGA ATGGCAAGGATGGCAACGAT | AAGGCTGCAGCGGCCGCTACTAGT TGACACCACACTTGCCGGGG | AAGGCTGCAGCGGCCGCTACTAGT ATGGCAAGGATGGCAACGAT | CCTTGAATTCGCGGCCGCATCTAGA TGACACCACACTTGCCGGGG |
| L. sativa LTP 1.2 | EF101531.1 | CCTTGAATTCGCGGCCGCATCTAGA GTGACATGTGGTCAGGTGGT | AAGGCTGCAGCGGCCGCTACTAGT CTGGATCCTATTGCAGTCGG | AAGGCTGCAGCGGCCGCTACTAGT GTGACATGTGGTCAGGTGGT | CCTTGAATTCGCGGCCGCATCTAGA CTGGATCCTATTGCAGTCGG |
| CCTTGAATTCGCGGCCGCATCTAGA | AAGGCTGCAGCGGCCGCTACTAGT | AAGGCTGCAGCGGCCGCTACTAGT | CCTTGAATTCGCGGCCGCATCTAGA |
