IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
(8 intermediate revisions by the same user not shown) | |||
Line 10: | Line 10: | ||
==Authors== | ==Authors== | ||
*[[User:Chloe T.C. Tang | TANG, Chloe T.C.]] | *[[User:Chloe T.C. Tang | TANG, Chloe T.C.]] | ||
* | * Tam, L.F. | ||
* | * Tam, K.M. | ||
* Mandy | * Tang, Mandy L.Y. | ||
* Mona | * Siu, Mona M.Y. | ||
* Yiu, Stephanie P.T. | * Yiu, Stephanie P.T. | ||
==Abstract== | ==Abstract== | ||
It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter (BBa_J23101) | It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in <em>Escherichia .coli</em> to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant. | ||
==Introduction== | ==Introduction== | ||
Line 33: | Line 33: | ||
==Conclusion== | ==Conclusion== | ||
Both the experimental cells and the control show a similar growth pattern and growth rate. It is concluded that either the experimental setup does not contain a strong enough promoter and ribosome binding site to create an environment of overloading cells; or overloading of cell do not affect its growth rate. | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | |||
__NOTOC__ | __NOTOC__ |
Revision as of 23:32, 27 January 2015
iGEM Project name 1 | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page | ||
|