IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions

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==Conclusion==
==Conclusion==


Both the experimental cells and the control show a similar growth pattern and growth rate. It is concluded that either the experimental setup does not contain a strong enough promoter and ribosome binding site to create an environment of overloading cells; or overloading of cell do not affect its growth rate.


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Revision as of 23:32, 27 January 2015

iGEM Project name 1 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page

Authors

  • TANG, Chloe T.C.
  • Tam, L.F.
  • Tam, K.M.
  • Tang, Mandy L.Y.
  • Siu, Mona M.Y.
  • Yiu, Stephanie P.T.

Abstract

It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in Escherichia .coli to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant.

Introduction

Methods and Materials

Results and Interpretations

Discussion

Conclusion

Both the experimental cells and the control show a similar growth pattern and growth rate. It is concluded that either the experimental setup does not contain a strong enough promoter and ribosome binding site to create an environment of overloading cells; or overloading of cell do not affect its growth rate.



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