IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
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==Abstract== | ==Abstract== | ||
It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in <em>Escherichia .coli</em> to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant. | It is hypothesized that strong gene expression will affect the growth and viability of cells. This experiment was conducted by incorporating a strong promoter from the Anderson Family (BBa_J23101: Registry of Standard Biological Parts) with a medium strength ribosome binding site (BBa_I13003: Registry of Standard Biological Parts) in <em>Escherichia .coli</em> (<em>E.coli</em>)to test the hypothesis. Quantitative results were obtained by optical density using a spectrophotometer with UV light of 600nm. The results indicated that the effect of overloading cells on growth rate was insignificant. | ||
==Introduction== | ==Introduction== |
Revision as of 00:16, 28 January 2015
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