IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
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Gene expression in bacteria involves the production of mRNA from DNA template and translation of the mRNA into protein. This two processes are driven by the binding of RNA polymerase to the promoter region of the gene and the binding of tRNA to the start codon nearest to the ribosome binding site (RBS) respectively. Therefore, to strengthen the expression of a foreign gene in ''E. coil'', strong promoters and RBS are selected as two controlling factors. | Gene expression in bacteria involves the production of mRNA from DNA template and translation of the mRNA into protein. This two processes are driven by the binding of RNA polymerase to the promoter region of the gene and the binding of tRNA to the start codon nearest to the ribosome binding site (RBS) respectively. Therefore, to strengthen the expression of a foreign gene in ''E. coil'', strong promoters and RBS are selected as two controlling factors. | ||
Under the stress of being transcribed too often, much energy and materials are required for gene expression. It is suspected that DNA overload will lower cell viability as energy and materials are wasted during gene expression. Also, the accumulation of proteins may perturb cellular function which in turn affect normal cell growth | Under the stress of being transcribed too often, much energy and materials are required for gene expression. It is suspected that DNA overload will lower cell viability as energy and materials are wasted during gene expression. Also, the accumulation of proteins may perturb cellular function which in turn affect normal cell growth. | ||
==Methods and Materials== | ==Methods and Materials== |
Revision as of 02:30, 28 January 2015
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