IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
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==Discussion== | ==Discussion== | ||
In our hypothesis, it is expected that strong gene expression will affect the growth and viability of cells. However, our results show no differences between the experimental setup with strong gene expression and the control in terms of growth rate. One possible explanation is that the promoter (BBa_J23101: Registry of Standard Biological Parts) and ribosome binding site (BBa_B0032: Registry of Standard Biological Parts) that we used in our experiments might not be strong enough and overloading of cells were not generated. Therefore, their growth rates show similar pattern. Another possible reason is that overloading of cells | In our hypothesis, it is expected that strong gene expression will affect the growth and viability of cells. However, our results show no differences between the experimental setup with strong gene expression and the control in terms of growth rate. One possible explanation is that the promoter (BBa_J23101: Registry of Standard Biological Parts) and ribosome binding site (BBa_B0032: Registry of Standard Biological Parts) that we used in our experiments might not be strong enough and overloading of cells were not generated. Therefore, their growth rates show similar pattern. Another possible reason is that overloading of cells might not have significant effects of its growth rate. Hence, it is hard to make concrete conclusion simply base on the results obtained. Further investigations are needed to test the hypothesis. | ||
==Conclusion== | ==Conclusion== |
Revision as of 02:35, 28 January 2015
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