IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
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Gene expression in bacteria involves the production of Messenger RNA (mRNA) from DNA template and translation of the mRNA into protein. These two processes are driven by the binding of RNA polymerase to the promoter region of the gene and the binding of Transfer RNA (tRNA) to the start codon nearest to the ribosome binding site (RBS) respectively. Therefore, to strengthen the expression of a foreign gene in ''E. coil'', strong promoters and RBS are selected as two controlling factors. | Gene expression in bacteria involves the production of Messenger RNA (mRNA) from DNA template and translation of the mRNA into protein. These two processes are driven by the binding of RNA polymerase to the promoter region of the gene and the binding of Transfer RNA (tRNA) to the start codon nearest to the ribosome binding site (RBS) respectively. Therefore, to strengthen the expression of a foreign gene in ''E. coil'', strong promoters and RBS are selected as two controlling factors. | ||
Under the stress of being transcribed too often, much energy and materials are required for gene expression. It is suspected that DNA overloading will lower cell viability as energy and materials are wasted during gene expression. Also, the accumulation of proteins may perturb cellular function which in turn | Under the stress of being transcribed too often, much energy and materials are required for gene expression. It is suspected that DNA overloading will lower cell viability as energy and materials are wasted during gene expression. Also, the accumulation of proteins may perturb cellular function which in turn affects normal cell growth.Therefore, we would like to investigate the effect of overloading on cell growth and viability. | ||
==Methods and Materials== | ==Methods and Materials== |
Revision as of 02:38, 28 January 2015
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