IGEM:Hong Kong HKUST/Investigations/Effect of overloading cells on growth rate/Entry Base: Difference between revisions
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==Discussion== | ==Discussion== | ||
According to | According to J. Monod ("The Growth of Bacterial Cultures"), a normal cell growth pattern, in theory, should shows a rapid growth at the beginning, then slows down to reach a steady state and die off at the end. However, all the experimental samples showed an unexpected growth pattern as they did not have a sign of reaching to a steady state. It is suggested that the number of measurements taken might not be enough for showing such a pattern. Also, after approximately 235 minutes, the growth rate of all the DNA samples suddenly increased and were not consistent with the previous rates. | ||
In our hypothesis, it is expected that strong gene expression will affect the growth and viability of cells. However, our results show no differences between the experimental setup with strong gene expression and the control in terms of growth rate. One possible explanation is that the promoter (BBa_J23101: Registry of Standard Biological Parts) and ribosome binding site (BBa_B0032: Registry of Standard Biological Parts) that we used in our experiments might not be strong enough and overloading of cells were not generated. Therefore, their growth rates show similar pattern. Another possible reason is that overloading of cells might not have significant effects of its growth rate. Hence, it is hard to make concrete conclusion simply base on the results obtained. Further investigations are needed to test the hypothesis. | In our hypothesis, it is expected that strong gene expression will affect the growth and viability of cells. However, our results show no differences between the experimental setup with strong gene expression and the control in terms of growth rate. One possible explanation is that the promoter (BBa_J23101: Registry of Standard Biological Parts) and ribosome binding site (BBa_B0032: Registry of Standard Biological Parts) that we used in our experiments might not be strong enough and overloading of cells were not generated. Therefore, their growth rates show similar pattern. Another possible reason is that overloading of cells might not have significant effects of its growth rate. Hence, it is hard to make concrete conclusion simply base on the results obtained. Further investigations are needed to test the hypothesis. |
Revision as of 07:30, 28 January 2015
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